Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 Potato Cyst Nematodes (Globodera rostochiensis) is one of the important potato’s pests and caused economic looses up to 70% in the several centrals of potato plantations in Indonesia.Potato Cyst Nematodes (PCN) shell component of egg shell containing chitin (inner layer) and vitelline/protein (outer layer), so the purpose of research was to fi nd out of chitin degrading bacteria for controlling of egg’s PCN by g fuel spiderverse cutting of their life cycle.The results showed that Bacillus sp.D2 isolated from potato rhizosphere could produce extra cellular chitinase in the medium containing of 0.
20% colloidal chitin and fermented for 72 hours.Result of chitinase purifi cation using ammonium sulphate precipitation and DEAE-Cellulose ion-exchange chromatography showed a specifi c activity 2691,052 U/mg and analyzing using SDS-PAGE 12.5% resulted in knipex 88 01 180 molecular weight 30 kDa.The apparent Km and Vmax of chitinase towards colloidal chitin were 2 mg/ml and 2.
2 μg/h, respectively. .